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                                                                                                                                                                                                                 7                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             *                           $                  	                           
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<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="3:48:41">2025.5.7</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="3:48:31">2025.5.7</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="3:45:51">2025.5.7</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="3:45:40">2025.5.7</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="3:42:53">2025.5.7</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>6ea803f5-7c1c-4a6c-a842-3a3e47c331cf</UUID>
<Type>Synthetic</Type>
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<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="3:42:45">2025.5.7</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>6ea803f5-7c1c-4a6c-a842-3a3e47c331cf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="3:39:28">2025.5.7</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>6ea803f5-7c1c-4a6c-a842-3a3e47c331cf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="3:39:15">2025.5.7</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>6ea803f5-7c1c-4a6c-a842-3a3e47c331cf</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="3:39:8">2025.5.7</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<UUID>c212cd1a-8e7f-4fb3-9f33-a82ec30a4c8b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="1:24:56">2025.1.14</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>c212cd1a-8e7f-4fb3-9f33-a82ec30a4c8b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="1:24:39">2025.1.14</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>c212cd1a-8e7f-4fb3-9f33-a82ec30a4c8b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="1:24:23">2025.1.14</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>c212cd1a-8e7f-4fb3-9f33-a82ec30a4c8b</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="1:24:9">2025.1.14</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="9:8:55">2025.1.10</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="5:27:8">2024.11.28</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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recentID="14" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5194-5315" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1803-1919" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7529-7633" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3624" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="4227" maxFusedRunOn="4227" detectionMode="exactProteinMatch"><Segment range="3574-3627" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EGRGSLLTCGDVEENPGP"/></Q></Feature><Feature recentID="18" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="5555-5561" color="#ffffff" type="standard"/><Segment range="5562-5579" color="#ffffff" type="standard"/><Segment name="-35" range="5580-5585" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5600-5621" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5507-5523" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5531-5547" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8107-8123" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2812-2821" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ×<Notes>
<UUID>747faf9a-94e3-4e6f-82c8-d87138f8e9b7</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pLV3-U6-Kdm7a(mouse)-shRNA1-CopGFP-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="3:53:23">2023.4.18</Created>
<LastModified UTC="2:3:20">2026.1.24</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO ÿþ              E              ÿÿÿÿYô? P                                                                                                                                                             e<StrandColors><TopStrand><ColorRange range="432..432" colors="red"/><ColorRange range="455..455" colors="red"/><ColorRange range="1128..1128" colors="red"/><ColorRange range="1318..1394" colors="black"/><ColorRange range="1395..1395" colors="red"/><ColorRange range="1396..1411" colors="black"/><ColorRange range="2817..3572" colors="black"/><ColorRange range="4622..4626" colors="red"/><ColorRange range="4637..4637" colors="red"/><ColorRange range="4802..4802" colors="red"/><ColorRange range="4915..4915" colors="red"/><ColorRange range="5302..5354" colors="purple"/><ColorRange range="5355..5407" colors="magenta"/><ColorRange range="5486..5489" colors="blue"/><ColorRange range="5490..5494" colors="black"/><ColorRange range="5506..5506" colors="blue"/><ColorRange range="6349..6349" colors="red"/></TopStrand><BottomStrand><ColorRange range="432..432" colors="red"/><ColorRange range="455..455" colors="red"/><ColorRange range="1128..1128" colors="red"/><ColorRange range="1318..1394" colors="black"/><ColorRange range="1395..1395" colors="red"/><ColorRange range="1396..1411" colors="black"/><ColorRange range="2817..3572" colors="black"/><ColorRange range="4622..4626" colors="red"/><ColorRange range="4637..4637" colors="red"/><ColorRange range="4802..4802" colors="red"/><ColorRange range="4915..4915" colors="red"/><ColorRange range="5302..5354" colors="purple"/><ColorRange range="5355..5407" colors="magenta"/><ColorRange range="5486..5489" colors="blue"/><ColorRange range="5490..5494" colors="black"/><ColorRange range="5506..5506" colors="blue"/><ColorRange range="6349..6349" colors="red"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
